中文姓名： 簡昆鎰

職稱：副教授

研究室名稱：蛋白質體分析實驗室

最高學歷：Ph.D.

學校/國家：清華大學/台灣

分機號碼：5971

電子郵件帳號： kychien@mail.cgu.edu.tw

個人網頁網址：

研究室現有： 博士後研究員  0 人

博士班研究生  2  人

碩士班研究生 0 人

專任研究助理  0  人

大學部專題生  0   人

研究方向及研究室特色：

蛋白質體學研究是指全面性的探討蛋白質表現程度的學門。在生物醫學領域中，通常著重於找出兩個樣品之間相對差異表現的蛋白。由於分析技術的快速進步，使得蛋白質體分析的覆蓋率，已逐漸趕上基因體學研究。其中最主要的兩項技術為質譜學及胜肽分離技術。本實驗室主要的研究興趣就是針對這兩項技術進行研發及改進，以提升蛋白質體數據的廣泛度、靈敏度、可信度及自動化程度。
對於胜肽分離系統的改進，我們建立了多種奈米級多維液相分離系統，不論是一般性的整體蛋白體分析，或是特定族群胜肽的純化分離，其效能均已達國際一流的水準。利用這些系統，我們可以從30ug的細胞蛋白萃取物中鑑定出6000-9000個蛋白，甚至可從200微克蛋白中鑑定出近萬條磷酸化胜肽。至於在質譜測量方面，我們著重於開發嶄新的同位素標定技術，以提高質譜定量數據的可靠性。目前我們已建立兩種標定技術，其中之ㄧ可進行整體蛋白體定量分析，另一則專注的定量特定胺基酸的氧化程度。這些技術都將運用於疾病蛋白體學研究，尤其將著重於分析臨床的組織或體液樣品。

最近五年所發表論文：

1. Tang WF, Huang RT, Chien KY, Huang JY, Lau KS, Jheng JR, Chiu CH, Wu TY, Chen CY, Horng JT. Host MicroRNA miR-197 Plays a Negative Regulatory Role in the Enterovirus 71 Infectious Cycle by Targeting the RAN Protein. J Virol. 2015 Nov 18;90(3):1424-38.

2. Geng J, Sun X, Wang P, Zhang S, Wang X, Wu H, Hong L, Xie C, Li X, Zhao H, Liu Q, Jiang M, Chen Q, Zhang J, Li Y, Song S, Wang HR, Zhou R, Johnson RL, Chien KY, Lin SC, Han J, Avruch J, Chen L, Zhou D. Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. Nat Immunol. 2015 Nov;16(11):1142-52.

3. Huang HW, Liu BS, Chien KY, Chiang LC, Huang SY, Sung WC, Wu WG. Cobra venom proteome and glycome determined from individual snakes of Naja atra reveal medically important dynamic range and systematic geographic variation. J Proteomics. 2015 Oct 14;128:92-104.

4. Chang KP, Lin SJ, Liu SC, Yi JS, Chien KY, Chi LM, Kao HK, Liang Y, Lin YT, Chang YS, Yu JS. Low-molecular-mass secretome profiling identifies HMGA2 and MIF as prognostic biomarkers for oral cavity squamous cell carcinoma. Sci Rep. 2015 Jul 3;5:11689.

5. Gopinath RK, You ST, Chien KY, Swamy KB, Yu JS, Schuyler SC, Leu JY. The Hsp90-dependent proteome is conserved and enriched for hub proteins with high levels of protein-protein connectivity. Genome Biol Evol. 2014 Oct 13;6(10):2851-65.

6. Chen CD, Wang CL, Yu CJ, Chien KY, Chen YT, Chen MC, Chang YS, Wu CC, Yu JS. Targeted proteomics pipeline reveals potential biomarkers for the diagnosis of metastatic lung cancer in pleural effusion. J Proteome Res. 2014 Jun 6;13(6):2818-29.

7. Lin SJ, Chang KP, Hsu CW, Chi LM, Chien KY, Liang Y, Tsai MH, Lin YT, Yu JS. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics. 2013 Dec 6;94:186-201.

8. Chang KP, Wang CL, Kao HK, Liang Y, Liu SC, Huang LL, Hseuh C, Hsieh YJ, Chien KY, Chang YS, Yu JS, Chi LM.Overexpression of caldesmon is associated with lymph node metastasis and poorer prognosis in patients with oral cavity squamous cell carcinoma. Cancer. 2013 Nov 15;119(22):4003-11.

9. Chen JT, Ho CW, Chi LM, Chien KY, Hsieh YJ, Lin SJ, Yu JS. Identification of the lamin A/C phosphoepitope recognized by the antibody P-STM in mitotic HeLa S3 cells. BMC Biochem. 2013 Jul 19;14:18.

10. Chen CL, Lin TS, Tsai CH, Wu CC, Chung T, Chien KY, Wu M, Chang YS, Yu JS, Chen YT. Identification of potential bladder cancer markers in urine by abundant-protein depletion coupled with quantitative proteomics. J Proteomics. 2013 Jun 24;85:28-43.

11. Dong YM, Chien KY, Chen JT, Lin SJ, Wang TC, Yu JS. Site-specific separation and detection of phosphopeptide isomers with pH-mediated stacking capillary electrophoresis-electrospray ionization-tandem mass spectrometry. J Sep Sci. 2013 May;36(9-10):1582-9.

12. Chen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT. Comparative and targeted proteomic analyses of urinary microparticles from bladder cancer and hernia patients. J Proteome Res. 2012 Dec 7;11(12):5611-29.

13. Wang CI, Chien KY, Wang CL, Liu HP, Cheng CC, Chang YS, Yu JS, Yu CJ. Quantitative proteomics reveals regulation of karyopherin subunit alpha-2 (KPNA2) and its potential novel cargo proteins in nonsmall cell lung cancer. Mol Cell Proteomics. 2012 Nov;11(11):1105-22.

14. Chen CY, Weng YH, Chien KY, Lin KJ, Yeh TH, Cheng YP, Lu CS, Wang HL. (G2019S) LRRK2 activates MKK4-JNK pathway and causes degeneration of SN dopaminergic neurons in a transgenic mouse model of PD. Cell Death Differ. 2012 Oct;19(10):1623-33.

15. Lin SY, Li TY, Liu Q, Zhang C, Li X, Chen Y, Zhang SM, Lian G, Liu Q, Ruan K, Wang Z, Zhang CS, Chien KY, Wu J, Li Q, Han J, Lin SC. Protein phosphorylation-acetylation cascade connects growth factor deprivation to autophagy. Autophagy. 2012 Sep;8(9):1385-6.

16. Hsieh YJ, Chien KY, Lin SY, Sabu S, Hsu RM, Chi LM, Lyu PC, Yu JS. Photofrin binds to procaspase-3 and mediates photodynamic treatment-triggered methionine oxidation and inactivation of procaspase-3. Cell Death Dis. 2012 Jul 12;3:e347.

17. Sun NK, Huang SL, Chien KY, Chao CC. Golgi-SNARE GS28 potentiates cisplatin-induced apoptosis by forming GS28-MDM2-p53 complexes and by preventing the ubiquitination and degradation of p53. Biochem J. 2012 Jun 1;444(2):303-14

18. Lin SY, Li TY, Liu Q, Zhang C, Li X, Chen Y, Zhang SM, Lian G, Liu Q, Ruan K, Wang Z, Zhang CS, Chien KY, Wu J, Li Q, Han J, Lin SC. GSK3-TIP60-ULK1 signaling pathway links growth factor deprivation to autophagy. Science. 2012 Apr 27;336(6080):477-81.

19. Feng Y, Chien KY, Chen HL, Chiu CH. Pseudogene recoding revealed from proteomic analysis of salmonella serovars. J Proteome Res. 2012 Mar 2;11(3):1715-9.

20. Tsai MH, Wu CC, Peng PH, Liang Y, Hsiao YC, Chien KY, Chen JT, Lin SJ, Tang RP, Hsieh LL, Yu JS. Identification of secretory gelsolin as a plasma biomarker associated with distant organ metastasis of colorectal cancer. J Mol Med (Berl).  2012 Feb;90(2):187-200.

21. Wu ZZ, Sun NK, Chien KY, Chao CC. Silencing of the SNARE protein NAPA sensitizes cancer cells to cisplatin by inducing ERK1/2 signaling, synoviolin ubiquitination and p53 accumulation. Biochem Pharmacol. 2011 Dec 1;82(11):1630-40.

22. Chang KP, Yu JS, Chien KY, Lee CW, Liang Y, Liao CT, Yen TC, Lee LY, Huang LL, Liu SC, Chang YS, Chi LM. Identification of PRDX4 and P4HA2 as metastasis-associated proteins in oral cavity squamous cell carcinoma by comparative tissue proteomics of microdissected specimens using iTRAQ technology. J Proteome Res. 2011 Nov 4;10(11):4935-47.