洪錦堂

職稱：教授兼科主任

研究室名稱：抗新冠病毒藥物研發實驗室

最高學歷：Ph.D.

學校/國家：英國劍橋大學

分機號碼：5156

電子郵件帳號：jimtong@mail.cgu.edu.tw

個人網頁網址：https://horng0866.wixsite.com/jimtong

研究室現有：博士後研究員1人

博士班研究生2人

碩士班研究生4人

專任研究助理3人

大學部專題生2人

Update：2022/08/03

研究方向及研究室特色：(2022.7更新)

研究題目: 1. Drug discovery against RNA viruses， 2. Molecular biology of RNA viruses

研究方向:

1.我們實驗室從事抗病毒植物新藥與小分子藥物的開發，希望從(1)臨床複方或單味植物新藥，(2)AI藥物開發，來開發與篩選具抗流行性感冒病毒、新冠病毒與腸病毒的抑制藥物，並研究其機轉，以因應可能爆發之疫情。

2. 利用定量蛋白質體學技術/高通量次世代定序，鑑定藥物之靶標蛋白質，透過miRNA/lncRNA表現差異，誘發訊號傳遞，了解宿主-病毒的相互調控，並研究其相關的抗病毒機制。

最近五年所發表論文：

1. Chang, Y. H., Lau, K. S., Kuo, R. L., and Horng*, J. T. (2017) dsRNA Binding Domain of PKR Is Proteolytically Released by Enterovirus A71 to Facilitate Viral Replication. Front Cell Infect Microbiol 7, 284 (IF:6.073, MICROBIOLOGY 33/136=24%)
2. Chen, S. G., Cheng, M. L., Chen, K. H., Horng, J. T., Liu, C. C., Wang, S. M., Sakurai, H., Leu, Y. L., Wang, S. D., and Ho, H. Y. (2017) Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo. Sci Rep 7, 935
3. Jheng, J. R., Lau, K. S., Lan, Y. W., and Horng, J. T. (2018) A novel role of ER stress signal transducer ATF6 in regulating enterovirus A71 viral protein stability. Journal of biomedical science 25, 9 (IF:12.771, MEDICINE, RESEARCH & EXPERIMENTAL 11/139=7.55%)
4. Huang, C. G., Lee, L. A., Wu, Y. C., Hsiao, M. J., Horng, J. T., Kuo, R. L., Huang, C. H., Lin, Y. C., Tsao, K. C., Chen, M. C., Chen, T. C., and Shih, S. R. (2018) A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients' responses to H7N9 infection. Oncotarget 9, 14492-14508
5. Sethy, B., Hsieh, C. F., Yeh, C., Horng, J. T., and Hsieh, P. W. (2018) Design, synthesis & structure-activity relationships of a new class of antihuman enterovirus D68 & A71 agents. Future Med Chem 10, 1333-1347
6. Tang, W. F., Huang, R. T., Chien, K. Y., Tang, P., and Horng*, J. T. (2018) Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71. Journal of proteome research (IF=5.370 BIOCHEMICAL RESEARCH METHODS 16/79=21%)
7. Sethy, B., Hsieh, C. F., Lin, T. J., Hu, P. Y., Chen, Y. L., Lin, C. Y., Tseng, S. N., Horng*, J. T., and Hsieh*, P. W. (2019) Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents. Journal of medicinal chemistry 62, 2390-2403 (IF=8.039, MEDICINAL CHEMISTRY 3/63=4 %)
8. Hsieh, C.F., Jheng, J.R., Lin, G.H., Chen, Y.L., Ho, J.Y., Liu, C.J., Hsu, K.Y., Chen, Y.S., Chan, Y.F., Yu, H.M., Hsieh, P.W., Chern, J.H., Horng*, J.T., 2020. Rosmarinic acid exhibits broad anti-enterovirus A71 activity by inhibiting the interaction between the five-fold axis of capsid VP1 and cognate sulfated receptors. Emerg Microbes Infect 9, 1194-1205. (IF=19.568, MICROBIOLOGY 7/136 =4.78%)
9. Chu, H.W., Sethy, B., Hsieh, P.W., and Horng*, J.T. (2021). Identification of Potential Drug Targets of Broad-Spectrum Inhibitors with a Michael Acceptor Moiety Using Shotgun Proteomics. Viruses 13. (IF=5.818, VIROLOGY 14/37 36%)
10. Tang, W.F., Tsai, H.P., Chang, Y.H., Chang, T.Y., Hsieh, C.F., Lin, C.Y., Lin, G.H., Chen, Y.L., Jheng, J.R., Liu, P.C., et al. (2021). Perilla (Perilla frutescens) leaf extract inhibits SARS-CoV-2 via direct virus inactivation. Biomed J 44, 293-303 (IF= 7.892, BIOCHEMISTRY & MOLECULAR BIOLOGY 47/296=15.71%)
11. Jheng, J.R., Chen, Y.S., and Horng*, J.T. (2021). Regulation of the proteostasis network during enterovirus infection: A feedforward mechanism for EV-A71 and EV-D68. Antiviral research 188, 105019. (IF=10.103, PHARMACOLOGY & PHARMACY 16/279 =5.56%)
12. Hsieh, C.F., Chen, Y.L., Lin, G.H., Chan, Y.F., Hsieh, P.W., and Horng*, J.T. (2022). 3,4-Dicaffeoylquinic Acid from the Medicinal Plant Ilex kaushue Disrupts the Interaction Between the Five-Fold Axis of Enterovirus A-71 and the Heparan Sulfate Receptor. Journal of virology 96, e0054221. (IF=6.549, VIROLOGY 11/37=29.7%)
13. Jheng, J.R., Hsieh, C.F., Chang, Y.H., Ho, J.Y., Tang, W.F., Chen, Z.Y., Liu, C.J., Lin, T.J., Huang, L.Y., Chern, J.H., et al. (2022). Rosmarinic acid interferes with influenza virus A entry and replication by decreasing GSK3beta and phosphorylated AKT expression levels. Journal of microbiology, immunology, and infection (in press) (IF=10.273, MICROBIOLOGY 17/136=12.5%)
14. Chin, Y.F., Tang, W.F., Chang, Y.H., Chang, T.Y., Lin, W.C., Lin, C.Y., Yang, C.M., Wu, H.L., Liu, P.C., Sun, J.R., et al. (2022). Orally delivered perilla (Perilla frutescens) leaf extract effectively inhibits SARS-CoV-2 infection in a Syrian hamster model. J Food Drug Anal 30, 252-270. (IF= 6.157, PHARMACOLOGY & PHARMACY 45/279 =15.95%)

執行中的計畫

Ongoing Research Support

MOST 109-2320-B-182 -026 -MY3   Horng (PI)     2020/08/01  ~   2023/07/31

衣康酸衍生物抑制流感病毒核酸蛋白出核的分子機轉研究

MOST 109-2634-F-182-001     Horng (co-PI)    2021/03/01  ~  2023/02/28

高教深根與特色中心計畫The Featured Areas Research Center Program within the framework of the Higher Education  Sprout Project by the Ministry of Education (MOE) in Taiwan and the Ministry of Science and Technology.

MOST 109-2327-B-182 -003 -   Horng (co-PI)       2022/03/01  ~   2026/02/28

防疫科學研究中心-長庚大學暨長庚醫院The Research Center for Epidemic Prevention Science