白麗美

職稱：教授

研究室名稱：發育學研究室

最高學歷：博士

學校/國家：University of North Carolina at Chapel Hill /USA

分機號碼：5520

電子郵件帳號： pai@mail.cgu.edu.tw

個人網頁網址：http://lmpflylab.wix.com/lmpflylab

研究室現有：博士後研究員3人

博士班研究生2人

碩士班研究生2人

專任研究助理3人

大學部專題生3人

update：2016/11/18

研究方向及研究室特色：

(1)   了解泛素化(ubiquitination)對於發育及癌細胞生長的角色

Ubiquitination屬於蛋白質後轉譯(post-translational) 調節的機制，主要由ubiquitin ligase決定那些蛋白受此機制調控。我們實驗室主要研究Cbl (E3 ligase)調節上皮生長激素(EGFR)，對果蠅體軸發育及癌細胞生長造成的影響。果蠅卵發育中Cbl 藉由影響EGFR的內噬及分解作用，而調降EGFR訊息傳遞，造成體軸的改變。人類癌細胞中Cbl也藉由此機制影響EGFR 訊息傳遞而抑制細胞生長。近期，我們發現果蠅Cbl影響CTP合成酶的聚合構造(CTP synthase filament)，而調節DNA的複製。Cbl 如何調解CTP合成酶的聚合構造，與此構造和癌症的關係是我們近期的研究方向。

(2)   代謝對果蠅再生與卵發育的影響

生物體常以調節代謝對外在環境作出快速反應。我們利用果蠅系統，了解蛋氨酸(Methionine)代謝促進果蠅卵發育，並藉由調節膜蛋白運輸而影響呼吸管的型態。同時，我們發現蛋氨酸(Methionine)代謝對果蠅翅膀再生產生影響。

蛋氨酸(Methionine)代謝如何影響蛋白運輸，及如何影響果蠅翅膀再生是我們近期的研究方向。

最近五年所發表論文：

1.    1. Yu-Hung Lin; Yen-Yi Zhen; I-Ching Lee; Ching-Wen Yang ; Li-Mei Pai*.

(2016) LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration. Under revision for Molecular Biology of Cell. SCI. IF=4.47

2.    Li-Mei Pai*, Pei-Yu Wang, Wei-Cheng Lin, Archan Chakraborty, Chau-Ting Yeh, Yu-Hung Lin. (2016. 4) Ubiquitination and filamentous structure of Cytidine triphosphate synthase. Fly. Invited review. SCI. IF=3.33

3.    Pei-Yu Wang, Wei-Cheng Lin, Yi-Cheng Tsai, Mei-Ling Cheng, Yu-Hung Lin, Shu-Heng Tseng, Archan Chakraborty, Li-Mei Pai*. (2015. 11) Regulation of CTP Synthase Filament Formation during DNA Endoreplication in Drosophila. Genetics. 201: 1511-1523. SCI. IF=5.96   

4.    Shiun Chang, Li-Mei Pai, Chien-Kuo Lee. (2015.10) In vitro generation of murine plasmacytoid Dendritic cells from lymphoid progenitors using the AC-6 feeder system. JoVE. SCI. IF=1.33

5.    Chia-Chun Chang, Wei-Cheng Lin, Li-Mei Pai, Hsuan-Shu Lee, Shinn-Chih Wu , Shih-Torng Ding, Ji-Long Liu*, Li-Ying Sung*.(2015.10) Cytoophidium assembly reflects upregulation of IMPDH activity. Journal of Cell Science. 128(19): 3550-3555. SCI. IF=5.43

6.    W-H Lee, P-Y Wang, Y-H Lin, H-Y Chou, Y-H Lee, C-K Lee, L-M Pai *. (2014. 12) Effects of RING-SH2Grb2, a Chimeric Protein Containing the E3 Ligase Domain of Cbl, on the EGFR Pathway. Chinese Journal of Physiology. 57(6):350-357. SCI. IF=1.16

7.    Gabriel N Aughey, Stuart J Grice, Qing-Ji Shen, Yichi Xu, Chia-Chun Chang, Ghows Azzam, Pei-Yu Wang, Luke Freeman-Mills, Li-Mei Pai, Li-Ying Sung, Jun Yan, Ji-Long Liu*. (2014.10) Nucleotide synthesis is regulated by cytoophidium formation during neurodevelopment and adaptive metabolism. Biology Open. 3(11): 1045-1056. SCI. IF=2.42

8.    Chou HY, Lin YH, Shiu GL, Tang HY, Cheng ML, Shiao MS, Pai LM*. (2014. 07) ADI1, a methionine salvage pathway enzyme, is required for Drosophila fecundity. J Biomed Sci. 21(1): 64-73. SCI. IF=2.76

9.    Yi-Cheng Tsai, Wei Chiang, Willisa Liou, Wei-Hao Lee, Yu-Wei Chang, Yi-Chen Li, Pei-Yu Wang, Tsubasa Tanaka, Akira Nakamura, and Li-Mei Pai*. (2014, 2) Endophilin B is required for Drosophila oocyte to endocytose yolk downstream of Oskar. Development. 141 (3):563-573. SCI. IF=6.46

10.      Yi-Ling Chen, Ting-Ting Chen, Li-Mei Pai, Joanna Wesoly, Hans A.R. Bluyssen, and Chien-Kuo Lee. (2013, 10) A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors. JEM. 210 (12), 2515-2522. SCI. IF=12.52

11.      Jen-Cheng Wang, Pei-Yu Wang, Hung-Ing Chen, Kai-Ling Wu, Li-Mei Pai, Tzer-En Nee*.(2013, 6) Lie Group Analysis of the Photo-Induced Fluorescence of Drosophila Oogenesis with the Asymmetrically Localized Gurken Protein. PLoSONE. 8:e65143. SCI. IF=3.23

12.      H Pi, YC Huang, IC Chen, CD Lin, HF Yeh, LM Pai. (2011, 06) Identification of 11-amino acid peptides that disrupt Notch-mediated processes in Drosophila. J Biomed Sci. 17; 18: 42-53. SCI. IF=2.76

13.      Pei-Yu Wang and Li-Mei Pai*. (2011.02) D-Cbl binding to Drk leads to dose-dependent down-regulation of EGFR signaling and increases receptor-ligand endocytosis. PLoSONE. 6(2) e17097. SCI. IF=3.23

14.      Jen-Cheng Wang, Pei-Yu Wang, Ruo-Rung Huang, Wei-Chieh Lin, Chia-Hui Fang, and Li-Mei Pai, Tzer-En Nee*. (2011. 02) Lie group study of Raman spectra of the Gurken gradient in Drosophila oogenesis. Anal. Bioanal. Chem. 400:335-341. SCI. IF=3.44